NuGlow® Copper Peptide with MD3 Copper Delivers More Copper Where It’s Needed Most.

NUGLOW MD3 (Micro-delivery) Copper complex stimulates collagen synthesis, angiogenesis and up-regulates those genes involved with stimulating and maintaining healthy young skin. Genes that increase hyaluronic acid, collagens and aquaporins.

Because NUGLOW MD3 Copper is only 60% of the size of GHK:CU and other copper formulations which has a significant impact on its potency:

1. For each gram of complex there are significantly more molecules present. This means that more copper can be delivered to the target cells. This is clearly demonstrated when looking at collagen induction. For the same concentration of complex (on a weight / volume basis) the NUGLOW MD3 induces significantly more collagen in fibroblasts.
2. Small molecules penetrate the skin much more efficiently and so a greater number of molecules reach the target cells. Being a smaller and simpler molecule GH is able to maintain its complex with copper more readily than GHK. This can be seen in the glycerin experiment in which GHK dissociates from CU causing a color change. NUGLOW MD3 is more stable and so acts as a much better carrier of copper to the skin.

In summary NUGLOW MD3is a smaller more potent delivery mechanism of copper to the skin which has been demonstrated by its increased ability to stimulate ECM elements such as collagen.

Study:

Copper Aminoacetylaminoimidazolyl Propanaoate – (MD3 COPPER)

Copper Aminoacetylaminoimidazolyl Propanaoate (MD3 COPPER) is a more active, stable and smaller copper complex than Prezatide copper complex (GHK:CU (2:1)). At 60% of the molecular weight of GHK:CU, MD3 COPPER has the potential to deliver more bioactivity and exhibit improved dermal penetration.

Improved activity can be demonstrated by the induction of collagen 1 in dermal fibroblasts. As seen in Figure 1 MD3 COPPER induces significantly more collagen 1 in normal human dermal fibroblasts (ATCC PCS-201-010) than GHK:CU. This was a blinded independent test performed by Sunny Bodiscovery (CA).

Figure 1: Induction of collagen 1 in fibroblasts

References

Improved stability can be demonstrated by the comparison of MD3 COPPER and GHK:CU when combined with glycerin, an widely used and important key ingredient of many skin care formulations. The GHK:CU complex breaks down resulting in a yellowing of the solution as opposed to MD3 COPPER which remains as an intact copper complex and maintains its characteristic blue color. This can been seen in Figure 2 below.

Figure 2: GHK:CU and MD3 COPPER in a 10% glycerin solution

Improved size characteristics means that the bulky GHK molecule of mw 340 has been reduced in size to just over 200 mw. This has provided a significant impact on cost and the ability of the complex to penetrate the skin. In addition there are more bioactive molecules per gram of material. However, despite these improvements the MD3 COPPER complex still provides the beneficial properties of GHK:CU. An example of this can be seen in the induction of angiogenesis as seen in Figure 3 in which both GHK:CU and MD3 COPPER induce angiogenesis as compared to a negative control and the positive control thymosin beta4.

Figure 3: Induction of angiogenesis

A second example, can be seen in a head to head comparison of gene array analysis of keratinocytes, looking at over 17,000 genes, where there was no significant difference in the up and down regulation profiles. Table 1 gives a snap shot of such genes.

Table 1: Microarray comparison for selected genes for cells exposed to GHK:CU and MD3 COPPER in human normal skin keratinocytes (ATCC CRL-2404)